Pulmonary CD103 expression regulates airway inflammation in asthma

Am J Physiol Lung Cell Mol Physiol. 2015 Apr 15;308(8):L816-26. doi: 10.1152/ajplung.00319.2014. Epub 2015 Feb 13.

Abstract

Although CD103(+) cells recently emerged as key regulatory cells in the gut, the role of CD103 ubiquitous expression in the lung and development of allergic airway disease has never been studied. To answer this important question, we evaluated the response of Cd103(-/-) mice in two separate well-described mouse models of asthma (ovalbumin and house dust mite extract). Pulmonary inflammation was assessed by analysis of bronchoalveolar lavage content, histology, and cytokine response. CD103 expression was analyzed on lung dendritic cells and T cell subsets by flow cytometry. Cd103(-/-) mice exposed to antigens developed exacerbated lung inflammation, characterized by increased eosinophilic infiltration, severe tissue inflammation, and altered cytokine response. In wild-type mice exposed to house dust mite, CD103(+) dendritic cells are increased in the lung and an important subset of CD4(+) T cells, CD8(+) T cells, and T regulatory cells express CD103. Importantly, Cd103(-/-) mice presented a deficiency in the resolution phase of inflammation, which supports an important role for this molecule in the control of inflammation severity. These results suggest an important role for CD103 in the control of airway inflammation in asthma.

Keywords: CD103; T regulatory cells; asthma; dendritic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / metabolism*
  • Asthma / immunology
  • Asthma / metabolism*
  • Cytokines / biosynthesis
  • Dendritic Cells / immunology
  • Gene Expression
  • Inflammation / metabolism
  • Integrin alpha Chains / genetics
  • Integrin alpha Chains / metabolism*
  • Lung / immunology
  • Lung / metabolism*
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD
  • Cytokines
  • Integrin alpha Chains
  • alpha E integrins