An amperometric biosensor for L-glutamate determination prepared from L-glutamate oxidase immobilized in polypyrrole-polyvinylsulphonate film

Artif Cells Nanomed Biotechnol. 2016;44(1):356-61. doi: 10.3109/21691401.2014.951723. Epub 2015 Feb 14.

Abstract

In this paper, a novel amperometric L-glutamate (Glu) biosensor with immobilization of L-glutamate oxidase (L-GlOx) on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been successfully developed. L-GlOx enzyme was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of Glu was carried out by oxidation of enzymatically produced H2O2 at 0.3 V versus Ag/AgCl. The optimum pH and temperature parameters were found to be 9.0 and 55 °C, respectively. There were three linear parts in the regions between 1.0 × 10(-9) and 1.0 × 10(-8) M (R(2) = 0.847), 5.0 × 10(-8) and 5.0 × 10(-7) M (R(2) = 0.997), 5.0 × 10(-7) and 5.0 × 10(-5) M (R(2) = 0.994). Storage stability, operation stability of the enzyme electrode were also studied.

Keywords: L-Glutamate; L-glutamate oxidase; biosensor; polypyrrole; polyvinylsulphonate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / chemistry*
  • Animals
  • Biosensing Techniques / methods*
  • Calibration
  • Cattle
  • Cross-Linking Reagents / chemistry
  • Electrochemical Techniques*
  • Electrodes
  • Enzymes, Immobilized
  • Glutamic Acid / blood*
  • Glutaral / chemistry
  • Humans
  • Hydrogen Peroxide / chemistry
  • Hydrogen-Ion Concentration
  • Polymers / chemistry*
  • Polyvinyls / chemistry*
  • Pyrroles / chemistry*
  • Sensitivity and Specificity
  • Serum Albumin, Bovine / chemistry
  • Sulfonic Acids / chemistry*
  • Temperature

Substances

  • Cross-Linking Reagents
  • Enzymes, Immobilized
  • Polymers
  • Polyvinyls
  • Pyrroles
  • Sulfonic Acids
  • lyapolate
  • Serum Albumin, Bovine
  • polypyrrole
  • Glutamic Acid
  • Hydrogen Peroxide
  • Amino Acid Oxidoreductases
  • L-glutamate oxidase
  • Glutaral