Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage-like cell line J774

J Mol Recognit. 2015 Apr;28(4):253-60. doi: 10.1002/jmr.2439. Epub 2015 Feb 12.

Abstract

WSXWS motif is a conserved amino acid sequence that is present in type I cytokine receptors. This motif that can be found both in the ligand binding chains and signal transducer molecule of the receptors with different amino acids at the position "X" plays a role in the receptor folding, ligand binding and signal transduction as well. Structural analysis proved that WSEWS motif of IL-6R is located in a highly accessible location in the protein. Structural properties and chemotaxis of a tetrapeptide library with SXWS sequence, where X was the 19 proteinogenic amino acids except cystein were systematically studied earlier. It has been proved that C-terminal amidation and the identity of amino acid X had a pronounced influence on the chemotactic properties but less of the structure of the peptides. Here, we present our findings on the effect of a tetrapeptide and a pentapeptide library with the sequence of SXWS and WSXWS on the chemotaxis and adhesion of J774 murine macrophage cell line. We studied the effect of the presence/absence of N-terminal tryptophan and the different amino acids at the X position on these physiological responses. Results indicated that amino acid X had a marked influence on chemotaxis, adhesion as well as on proliferation induced by (W)SXWS peptides. Elongation of SXWS sequence with a tryptophan at the N terminus also altered pronouncedly all the physiological responses of the cells studied. A good correlation could be observed between the chemotaxis and the proliferation and physicochemical parameters of the amino acid X.

Keywords: IL-6R peptides; J774 cells; adhesion; chemotaxis; physicochemical parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Line
  • Chemotaxis*
  • Cytokine Receptor gp130 / metabolism
  • Macrophages / cytology*
  • Macrophages / metabolism*
  • Mice
  • Peptides / metabolism*

Substances

  • Il6st protein, mouse
  • Peptides
  • Cytokine Receptor gp130