Abstract
A novel and label-free fluorescence assay for histone acetyltransferase (HAT) activity was established via in situ generation of a nucleic acid-mimicking CoA-Au(I) coordination polymer (CP). Moreover, the potency of this assay for HAT-targeted drug discovery was proved by screening HAT inhibitors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomimetic Materials / chemistry*
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Coenzyme A / chemistry
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Fluorescent Dyes / chemistry*
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Gold / chemistry
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Histone Acetyltransferases / metabolism*
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Nucleic Acids / chemistry
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Organic Chemicals / chemistry
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Polymers / chemistry*
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Spectrometry, Fluorescence
Substances
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Fluorescent Dyes
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Nucleic Acids
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Organic Chemicals
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Polymers
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SYBR Green II
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Gold
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Histone Acetyltransferases
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Coenzyme A