The characteristics of the patellar tendon reflex provide fundamental insight regarding the diagnosis of neurological status. Based on the features of the tendon reflex response, a clinician may establish preliminary perspective regarding the global condition of the nervous system. Current techniques for quantifying the observations of the reflex response involve the application of ordinal scales, requiring the expertise of a highly skilled clinician. However, the reliability of the ordinal scale approach is debatable. Highly skilled clinicians have even disputed the presence of asymmetric reflex pairs. An alternative strategy was the implementation of an iPod wireless accelerometer application to quantify the reflex response acceleration waveform. An application enabled the recording of the acceleration waveform and later wireless transmission as an email attachment by connectivity to the Internet. A potential energy impact pendulum enabled the patellar tendon reflex to be evoked in a predetermined and targeted manner. Three feature categories of the reflex response acceleration waveform (global parameters, temporal organization, and spectral features) were incorporated into machine learning to distinguish a subject's hemiplegic and healthy reflex pair. Machine learning attained perfect classification of the hemiplegic and healthy reflex pair. The research findings implicate the promise of machine learning for providing increased diagnostic acuity regarding the acceleration waveform of the tendon reflex response.