Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells

Cell. 1989 Jul 28;58(2):293-303. doi: 10.1016/0092-8674(89)90844-1.


Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum- antigens) recognized by cytolytic T cells. We found that the gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence. The sequence shows no significant similarity with sequences in current data bases. A mutation that causes expression of the antigen is located in exon 4; it is the only apparent difference between the normal and the antigenic alleles. A short synthetic peptide corresponding to a region of exon 4 located around this mutation makes P815 cells sensitive to lysis by anti-P91A cytolytic T cells. The mutation creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule. Several secondary tumor cell variants that no longer express tum- antigen P91A were found to carry deletions in the gene.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / metabolism
  • Base Sequence
  • Cell Line
  • Exons*
  • Genetic Variation
  • H-2 Antigens / immunology*
  • Histocompatibility Antigens / genetics*
  • Histocompatibility Antigens / immunology
  • Histocompatibility Antigens / metabolism
  • Mice
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • Suppression, Genetic*
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Tumor Cells, Cultured / immunology
  • Tumor Cells, Cultured / metabolism


  • Antigens, Neoplasm
  • H-2 Antigens
  • Histocompatibility Antigens
  • transplantation antigen P91A, mouse

Associated data

  • GENBANK/M25149
  • GENBANK/M25150