MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing

Nat Protoc. 2015 Mar;10(3):475-83. doi: 10.1038/nprot.2014.114. Epub 2015 Feb 18.


Current high-throughput DNA sequencing technologies enable acquisition of billions of data points through which myriad biological processes can be interrogated, including genetic variation, chromatin structure, gene expression patterns, small RNAs and protein-DNA interactions. Here we describe the MethylC-sequencing (MethylC-seq) library preparation method, a 2-d protocol that enables the genome-wide identification of cytosine DNA methylation states at single-base resolution. The technique involves fragmentation of genomic DNA followed by adapter ligation, bisulfite conversion and limited amplification using adapter-specific PCR primers in preparation for sequencing. To date, this protocol has been successfully applied to genomic DNA isolated from primary cell culture, sorted cells and fresh tissue from over a thousand plant and animal samples.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cytosine / chemistry*
  • DNA Fragmentation
  • DNA Methylation / genetics*
  • Genomic Library*
  • Genomics / methods*
  • Sequence Analysis, DNA / methods*
  • Sulfites / chemistry


  • Sulfites
  • Cytosine
  • hydrogen sulfite