Structure of a left-handed DNA G-quadruplex

Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):2729-33. doi: 10.1073/pnas.1418718112. Epub 2015 Feb 18.

Abstract

Aside from the well-known double helix, DNA can also adopt an alternative four-stranded structure known as G-quadruplex. Implications of such a structure in cellular processes, as well as its therapeutic and diagnostic applications, have been reported. The G-quadruplex structure is highly polymorphic, but so far, only right-handed helical forms have been observed. Here we present the NMR solution and X-ray crystal structures of a left-handed DNA G-quadruplex. The structure displays unprecedented features that can be exploited as unique recognition elements.

Keywords: G-quadruplex; NMR; X-ray crystallography; left-handed helix; nucleic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray
  • DNA / chemistry*
  • Magnetic Resonance Spectroscopy
  • Nucleic Acid Conformation*

Substances

  • DNA

Associated data

  • PDB/2MS9
  • PDB/4U5M