MultiPLX: automatic grouping and evaluation of PCR primers

Lauris Kaplinski; Maido Remm

doi:10.1007/978-1-4939-2365-6_9

MultiPLX: automatic grouping and evaluation of PCR primers

Methods Mol Biol. 2015:1275:127-42. doi: 10.1007/978-1-4939-2365-6_9.

Authors

Lauris Kaplinski¹, Maido Remm

Affiliation

¹ Department of Bioinformatics, University of Tartu, Tartu, Estonia.

PMID: 25697656
DOI: 10.1007/978-1-4939-2365-6_9

Abstract

In this chapter we describe MultiPLX-a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from http://bioinfo.ut.ee/?page_id=167. Graphical web-based interface to most functions of MultiPLX is available at http://bioinfo.ut.ee/multiplx/.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Primers / genetics*
Humans
Polymerase Chain Reaction / methods*

Substances

DNA Primers