Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin

Stem Cell Reports. 2015 Mar 10;4(3):503-18. doi: 10.1016/j.stemcr.2015.01.015. Epub 2015 Feb 19.


Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin(-)SCA-1(+)CD49f(+)TROP2(high) phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin(-)CD49f(+)TROP2(high) PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Culture Techniques*
  • Cell Differentiation
  • Cell Self Renewal
  • Cell Separation* / methods
  • Culture Media, Serum-Free
  • Gene Expression Profiling
  • Humans
  • Immunomagnetic Separation / methods
  • Male
  • Mice
  • NF-kappa B / metabolism
  • Progesterone / metabolism
  • Progesterone / pharmacology
  • Prostate / cytology*
  • Prostate / metabolism
  • Proto-Oncogene Proteins c-myc / metabolism
  • Signal Transduction
  • Sodium Selenite / pharmacology
  • Stem Cells / cytology*
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • Transcriptome
  • Tumor Necrosis Factor-alpha / metabolism


  • Culture Media, Serum-Free
  • NF-kappa B
  • Proto-Oncogene Proteins c-myc
  • Tumor Necrosis Factor-alpha
  • Progesterone
  • Sodium Selenite

Associated data

  • GEO/GSE61861