Investigation of the effects of distance from sources on apoptosis, oxidative stress and cytosolic calcium accumulation via TRPV1 channels induced by mobile phones and Wi-Fi in breast cancer cells

Biochim Biophys Acta. 2015 Oct;1848(10 Pt B):2756-65. doi: 10.1016/j.bbamem.2015.02.013. Epub 2015 Feb 19.

Abstract

TRPV1 is a Ca2+ permeable channel and gated by noxious heat, oxidative stress and capsaicin (CAP). Some reports have indicated that non-ionized electromagnetic radiation (EMR)-induces heat and oxidative stress effects. We aimed to investigate the effects of distance from sources on calcium signaling, cytosolic ROS production, cell viability, apoptosis, plus caspase-3 and -9 values induced by mobile phones and Wi-Fi in breast cancer cells MCF-7 human breast cancer cell lines were divided into A, B, C and D groups as control, 900, 1800 and 2450 MHz groups, respectively. Cells in Group A were used as control and were kept in cell culture conditions without EMR exposure. Groups B, C and D were exposed to the EMR frequencies at different distances (0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm) for 1h before CAP stimulation. The cytosolic ROS production, Ca2+ concentrations, apoptosis, caspase-3 and caspase-9 values were higher in groups B, C and D than in A group at 0 cm, 1 cm and 5 cm distances although cell viability (MTT) values were increased by the distances. There was no statistically significant difference in the values between control, 20 and 25 cm. Wi-Fi and mobile phone EMR placed within 10 cm of the cells induced excessive oxidative responses and apoptosis via TRPV1-induced cytosolic Ca2+ accumulation in the cancer cells. Using cell phones and Wi-Fi sources which are farther away than 10 cm may provide useful protection against oxidative stress, apoptosis and overload of intracellular Ca2+. This article is part of a Special Issue entitled: Membrane channels and transporters in cancers.

Keywords: Apoptosis; Breast cancer; Calcium signaling; Mobile phone; Oxidative stress; Wireless internet (Wi-Fi).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / radiation effects
  • Calcium / metabolism*
  • Calcium Signaling
  • Cell Phone
  • Cell Survival / radiation effects
  • Cytosol / enzymology
  • Cytosol / radiation effects
  • Dose-Response Relationship, Radiation
  • Electromagnetic Radiation*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MCF-7 Cells
  • Oxidative Stress / radiation effects
  • Reactive Oxygen Species / agonists
  • Reactive Oxygen Species / metabolism
  • TRPV Cation Channels / agonists*
  • TRPV Cation Channels / genetics
  • TRPV Cation Channels / metabolism

Substances

  • Reactive Oxygen Species
  • TRPV Cation Channels
  • TRPV1 protein, human
  • Calcium