DNA electrochemical biosensor for metallic drugs at physiological conditions

Angel J Santiago-Lopez; José L Vera; Enrique Meléndez

doi:10.1016/j.jelechem.2014.07.022

DNA electrochemical biosensor for metallic drugs at physiological conditions

J Electroanal Chem (Lausanne). 2014 Oct 1:731:139-144. doi: 10.1016/j.jelechem.2014.07.022.

Authors

Angel J Santiago-Lopez¹, José L Vera², Enrique Meléndez²

Affiliations

¹ University of Puerto Rico, Department of Chemical Engineering, PO Box 9019 Mayagüez, PR 00681.
² University of Puerto Rico, Department of Chemistry, PO Box 9019 Mayagüez, PR 00681.

Abstract

Entrapment of dsSS-DNA into the polypyrrole-polyvinyl sulphonate (dsSS-DNA-PPy-PVS) film over indium-tin-oxide (ITO) coated glass has been designed to detect titanium and platinum drugs, titanocene dichloride and cisplatin. The disposable dsSS-DNA-PPy-PVS/ITO biosensor was characterized by cyclic voltammetry, attenuated total reflectance Infrared spectroscopy and atomic force microscopy. Amperometric studies by cyclic voltammetry using, dsSS-DNA-PPy PVS/ITO biosensor, demonstrated the ability of this biosensor to detect these metallic drugs in millimolar concentration by monitoring the decrease of the guanine oxidation signal as a result of the DNA damage. The concentration range detected for titanocene dichloride is 0.25 to 1.5 mM and for cisplatin is 0.06 to 1.0 mM.

Keywords: DNA biosensor; cisplatin; electrochemical detection; electrochemical entrapment; titanocene dichloride.

Grants and funding

R25 GM088023/GM/NIGMS NIH HHS/United States