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. 2014 Jun;9(2):162-7.

Detection of highly ciprofloxacin resistance acinetobacter baumannii isolated from patients with burn wound infections in presence and absence of efflux pump inhibitor

Mohammad-Hossein Maleki  1 , Farid Azizi Jalilian  2 , Hatef Khayat  3 , Maryam Mohammadi  3 , Fazel Pourahmad  4 , Khairollah Asadollahi  5 , Iraj Pakzad  2 , Nourkhoda Sadeghifard  2 , Setareh Soroush  2 , Mohammad Emaneini  6 , Morovat Taherikalani  2
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Detection of highly ciprofloxacin resistance acinetobacter baumannii isolated from patients with burn wound infections in presence and absence of efflux pump inhibitor

Mohammad-Hossein Maleki et al. Maedica (Bucur). 2014 Jun.

Abstract

The emergence of fluoroquinolone resistance among A. baumannii isolates is now of particular concern. Phenotypic and genotypic characteristics of resistance to ciprofloxacin among 50 Acinetobacter baumannii isolated from burn wound infections of Tehran were evaluated by E-test and broth microdilution in presence and absence of efflux pump inhibitor phenylalanine- arginine β-naphthylamide (PAβN) and PCR-sequencing methods. All isolates were then typed by REP-PCR fingerprinting to find the clonal relationship between resistant isolates. Our results indicated that resistance to ciprofloxacin among A. baumannii isolated from burn infections in Tehran are high with resistance rate of 100% and ciprofloxacin resistant isolates have a mutation of Serine 83 →Leucine in the quinolone resistance determining region (QRDR) of DNA gyrase subunit A (GyrA). 38% of the isolates showed MIC ranges of 64 to ≥512μg/ml and were considered as highly resistant. We could not detect Par C mutations and plasmid-mediated quinolone resistance A (qnrA) among ciprofloxacin resistant isolates. When we used the efflux pump inhibitor PAbN, MIC of ciprofloxacin was reduced two-to four folds. REP-type A (25/50; 50%), B (20/50; 30%) and C (10/50; 20%) were the most common REP-types among A. baumannii isolates. It seems that mutation in GyrA is the main mechanism of resistant to ciprofloxacin among A. baumannii isolates from burn infections and presence of efflux pumps is just secondary target for ciprofloxacin resistant among A. baumannii in Iran. Regarding with limitation of REP-types detected in this study, we found good correlation between resistance to ciprofloxacin and REP-types A-C.

Keywords: A. baumannii; ciprofloxacin resistance; gyr A QRDRS.

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Figures

Dendrogram 1
Dendrogram 1. Distribution of REP types A to C among 19 highly ciprofloxacin resistant A. baumannii isolated from burn wound infections and their clonal relationship.
See this image and copyright information in PMC

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References

    1. Oncul O, Oksuz S, Acar A, et al. Nosocomial infection characteristics in a burn intensive care unit: Analysis of an eleven-year active surveillance. Burns. 2014;40:835–41. - PubMed
    1. Lee HG, Jang J, Choi JE, et al. Blood stream infections in patients in the burn intensive care unit. Infect Chemother. 2013;45:194–201. - PMC - PubMed
    1. Hujer KM, Hujer AM, Hulten EA, et al. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemother. 2006;50:4114–23. - PMC - PubMed
    1. Asadollahi K, Alizadeh E, Akbari M, et al. The role of bla(OXA-like carbapenemase) and their insertion sequences (ISS) in the induction of resistance against carbapenem antibiotics among Acinetobacter baumannii isolates in Tehran hospitals. Rouman Arch Microbiol Immunol. 2011;70:153–8. - PubMed
    1. Asadollahi K, Taherikalani M, Maleki A, et al. Diversity of aminoglycoside modifying enzyme genes among multidrug resistant Acinetobacter baumannii genotypes isolated from nosocomial infections in Tehran hospitals and their association with class 1 integrons. Acta Microbiol Immunol Hung. 2011;58:359–70. - PubMed

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