The distribution of somatostatin-containing neurons in mice of both sexes was immunohistochemically examined and compared with that in rats. In radioimmunoassay the relative somatostatin content in the mouse brain was 2-3 times higher than that in the rat. The overall immunohistochemical staining for somatostatin was much stronger and more prominent in the mouse than in the rat. Although the distribution pattern of somatostatin immunoreactivity was basically the same between the two animals, several regions, especially the nucleus anterior hypothalami and the nucleus interpeduncularis, were found to contain large aggregates of somatostatin-immunoreactive neurons in the mouse brain but not in the rat. The electrolytic lesions to the nucleus anterior hypothalami caused a marked decrease in somatostatin immunoreactivity of the outer layer of the median eminence in the mouse. This suggests that the nucleus anterior hypothalami is an additional source of somatostatin for the median eminence in the mouse. The differences recognized between the species are interesting from functional and evolutionary points of view.