L(+)-2-amino-4-phosphonobutyrate inhibits the release of both glutamate and dynorphin from guinea pig but not rat hippocampal mossy fiber synaptosomes

Brain Res. 1989 Aug 21;495(1):151-5. doi: 10.1016/0006-8993(89)91229-8.

Abstract

The K+-evoked release of dynorphin A(1-8)-like immunoreactivity from guinea pig hippocampal mossy fiber synaptosomes was inhibited 53% by L(+)-2-amino-4-phosphonobutyrate (L(+)APB, 300 microM), a glutamate analogue. Equimolar L(+)APB also inhibited the Ca2+-dependent component of endogenous L-glutamate release from these mossy fiber synaptosomes by 40%. The K+-evoked release of both glutamate and dynorphin A(1-8) from rat hippocampal mossy fiber synaptosomes were unaffected by L(+)APB. It is proposed that L(+)APB selectively suppresses the excitatory mossy fiber synaptic inhibiting the Ca2+-dependent release of glutamate and dynorphin A(1-8) from guinea pig but not rat hippocampal mossy fiber terminals.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aminobutyrates / pharmacology*
  • Animals
  • Dynorphins / metabolism*
  • Glutamates / metabolism*
  • Glutamic Acid
  • Guinea Pigs
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • In Vitro Techniques
  • Male
  • Peptide Fragments / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*

Substances

  • Aminobutyrates
  • Glutamates
  • Peptide Fragments
  • Glutamic Acid
  • Dynorphins
  • dynorphin (1-8)
  • 2-amino-4-phosphonobutyric acid