GlycanAnalysis Plug-in: a database search tool for N-glycan structures using mass spectrometry

Bioinformatics. 2015 Jul 1;31(13):2217-9. doi: 10.1093/bioinformatics/btv110. Epub 2015 Feb 23.


Tandem mass spectrometry (MS/MS or MS(n)) is a potent technique for characterizing N-glycan structures. GlycanAnalysis searches a glycan database to support the identification of glycan structures from MS/MS spectra. It also calculates diagnostic ions of glycan structures registered in a glycan database (GlycomeDB or KEGG GLYCAN) and searches for MS/MS spectra of N-glycans that match diagnostic ions to determine the structures. This program functions as a plug-in for Mass++, a freeware mass spectrum visualization and analysis program.

Availability and implementation: The executable files of Mass++ are available for free at The GlycanAnalysis plug-in is included in the standard package of Mass++ for Windows.

Contact: or or

Supplementary information: Supplementary material are available at Bioinformatics online.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Databases, Factual*
  • Glycopeptides / analysis*
  • Glycopeptides / chemistry
  • Glycosylation
  • Humans
  • Mass Spectrometry
  • Polysaccharides / analysis*
  • Polysaccharides / chemistry
  • Proteomics / methods
  • Search Engine*
  • Software*
  • Tandem Mass Spectrometry / methods*


  • Glycopeptides
  • Polysaccharides