Target specificity of the CRISPR-Cas9 system

Quant Biol. 2014 Jun;2(2):59-70. doi: 10.1007/s40484-014-0030-x.


The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

Keywords: CRISPR; Cas9; genome engineering; off-targets; target specificity.