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, 16 (1), 43

Fate by RNA Methylation: m6A Steers Stem Cell Pluripotency


Fate by RNA Methylation: m6A Steers Stem Cell Pluripotency

Boxuan Simen Zhao et al. Genome Biol.


The N 6-methyladenosine (m6A) modification of mRNA has a crucial function in regulating pluripotency in murine stem cells: it facilitates resolution of naïve pluripotency towards differentiation.


Figure 1
Figure 1
The establishment and role of m 6 A RNA methylation in development. (a) RNA methylation is dynamically regulated and impacts various aspects of RNA metabolism. The m6A marker is created by the activity of the m6A methyltransferase complex (METTL3-METTL14), and the methyl group can be reversibly removed by m6A demethylases; the modification can be recognized by m6A-binding proteins to effect biological functions. (b) Divergent effects of depletion through knockout (KO) of the N6-adenosine-methyltransferase subunit METTL3 during naïve and primed pluripotent states. As m6A reduces the stability of methylated transcripts, depletion of METTL3 in naïve pluripotent cells further upregulates the already-high naïve pluripotency genes to create a ‘hyper’-naïve pluripotent state, whereas depletion during the primed state further boosts the dominating lineage-commitment factors and tips the balance towards differentiation.

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