Structural studies of O-polysaccharide isolated from Cronobacter sakazakii Sequence Type 12 from a case of neonatal necrotizing enterocolitis

Carbohydr Res. 2015 Apr 30;407:55-8. doi: 10.1016/j.carres.2015.01.020. Epub 2015 Jan 31.

Abstract

The O-polysaccharide (OPS) of Cronobacter sakazakii NTU 696 (Sequence Type 12) from a case of neonatal necrotizing enterocolitis was isolated from the polysaccharide fraction obtained after lipopolysaccharide (LPS) hydrolysis. Purified OPS was analyzed by NMR spectroscopy ((1)H, COSY, TOCSY, NOESY, HSQC, HSQC-TOCSY and HMBC experiments) and chemical methods. Obtained monosaccharide derivatives analyzed by gas chromatography and gas chromatography-mass spectrometry allowed the identification of six sugar components. Performed experiments enabled to establish a structure of the OPS repeating unit of C. sakazakii NTU 696, as: [structure: see text].

Keywords: Cronobacter sakazakii; NMR; O-polysaccharide; Structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Cronobacter sakazakii / chemistry
  • Cronobacter sakazakii / isolation & purification*
  • Cronobacter sakazakii / metabolism
  • Enterobacteriaceae Infections / microbiology*
  • Enterocolitis, Necrotizing / microbiology*
  • Humans
  • Infant, Newborn
  • Magnetic Resonance Spectroscopy
  • O Antigens / chemistry*
  • O Antigens / isolation & purification

Substances

  • O Antigens