The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells

FEBS Lett. 2015 Apr 2;589(8):924-32. doi: 10.1016/j.febslet.2015.02.022. Epub 2015 Feb 26.


The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

Keywords: 5′-UTR; Isoform; RPD3; UME6; VTH1; VTH2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / genetics
  • Acetates / pharmacology*
  • Base Sequence
  • Enzyme Induction / drug effects
  • Fermentation*
  • Gene Expression Regulation, Fungal
  • Histone Deacetylases / metabolism*
  • Isoenzymes / metabolism
  • Meiosis
  • Mutation
  • Promoter Regions, Genetic / genetics
  • RNA, Untranslated / genetics
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / biosynthesis*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Vesicular Transport Proteins / biosynthesis*
  • Vesicular Transport Proteins / genetics


  • 5' Untranslated Regions
  • Acetates
  • Isoenzymes
  • PEP1 protein, S cerevisiae
  • RNA, Untranslated
  • Repressor Proteins
  • SIN3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • UME6 protein, S cerevisiae
  • Vesicular Transport Proteins
  • RPD3 protein, S cerevisiae
  • Histone Deacetylases