Antimicrobial Efficacy of an Innovative Emulsion of Medium Chain Triglycerides Against Canine and Feline Periodontopathogens

J Small Anim Pract. 2015 Apr;56(4):253-63. doi: 10.1111/jsap.12344. Epub 2015 Feb 28.

Abstract

Objectives: To test the in vitro antimicrobial efficacy of a non-toxic emulsion of free fatty acids against clinically relevant canine and feline periodontopathogens

Methods: Antimicrobial kill kinetics were established utilising an alamarBlue(®) viability assay against 10 species of canine and feline periodontopathogens in the biofilm mode of growth at a concentration of 0·125% v/v medium chain triglyceride (ML:8) emulsion. The results were compared with 0·12% v/v chlorhexidine digluconate and a xylitol-containing dental formulation. Mammalian cellular cytotoxicity was also investigated for both the ML:8 emulsion and chlorhexidine digluconate (0·25 to 0·0625% v/v) using in vitro tissue culture techniques.

Results: No statistically significant difference was observed in the antimicrobial activity of the ML:8 emulsion and chlorhexidine digluconate; a high percentage kill rate (>70%) was achieved within 5 minutes of exposure and was maintained at subsequent time points. A statistically significant improvement in antibiofilm activity was observed with the ML:8 emulsion compared with the xylitol-containing formulation. The ML:8 emulsion possessed a significantly lower (P < 0·001) toxicity profile compared with the chlorhexidine digluconate in mammalian cellular cytotoxicity assays.

Clinical significance: The ML:8 emulsion exhibited significant potential as a putative effective antimicrobial alternative to chlorhexidine- and xylitol- based products for the reduction of canine and feline periodontopathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Infective Agents / administration & dosage
  • Anti-Infective Agents / pharmacology*
  • Biofilms / drug effects
  • Cat Diseases / microbiology
  • Cat Diseases / prevention & control*
  • Cats
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / analogs & derivatives
  • Chlorhexidine / pharmacology
  • Dog Diseases / microbiology
  • Dog Diseases / prevention & control*
  • Dogs
  • Emulsions
  • Fibroblasts / drug effects
  • Gram-Negative Bacteria / drug effects
  • Gram-Positive Bacteria / drug effects
  • Microbial Sensitivity Tests / veterinary
  • Periodontitis / microbiology
  • Periodontitis / prevention & control
  • Periodontitis / veterinary*
  • Triglycerides / administration & dosage
  • Triglycerides / pharmacology*

Substances

  • Anti-Infective Agents
  • Emulsions
  • Triglycerides
  • chlorhexidine gluconate
  • Chlorhexidine