Genome sequence-independent identification of RNA editing sites

Nat Methods. 2015 Apr;12(4):347-50. doi: 10.1038/nmeth.3314. Epub 2015 Mar 2.


RNA editing generates post-transcriptional sequence changes that can be deduced from RNA-seq data, but detection typically requires matched genomic sequence or multiple related expression data sets. We developed the GIREMI tool (genome-independent identification of RNA editing by mutual information; to predict adenosine-to-inosine editing accurately and sensitively from a single RNA-seq data set of modest sequencing depth. Using GIREMI on existing data, we observed tissue-specific and evolutionary patterns in editing sites in the human population.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Evolution, Molecular
  • Genomics*
  • Humans
  • RNA Editing / genetics*
  • Sequence Analysis, RNA / methods*