Simultaneous generation of many RNA-seq libraries in a single reaction

Nat Methods. 2015 Apr;12(4):323-5. doi: 10.1038/nmeth.3313. Epub 2015 Mar 2.


Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics
  • Base Sequence*
  • Gene Expression Profiling / standards
  • Gene Library*
  • Sequence Analysis, RNA / economics
  • Sequence Analysis, RNA / methods*
  • Sequence Analysis, RNA / standards
  • Time Factors