Rhodamine-123: a p-glycoprotein marker complex with sodium lauryl sulfate

Pak J Pharm Sci. 2015 Mar;28(2):617-22.

Abstract

Aim of this study was to investigate the role of sodium lauryl sulfate (SLS) as P-glycoprotein inhibitor. The everted rat gut sac model was used to study in-vitro mucosal to serosal transport of Rhodamine-123 (Rho-123). Surprisingly, SLS decreases the serosal absorption of Rho-123 at all investigated concentrations. Investigation reveals complex formation between Rhodamine-123 and sodium lauryl sulfate. Interaction profile of SLS & Rho-123 was studied at variable SLS concentrations. The SLS concentration higher than critical micelle concentration (CMC) increases the solubility of Rho-123 but could not help in serosal absorption, on the contrary the absorption of Rho-123 decreased. Rho-123 and SLS form pink color complex at sub-CMC. The SLS concentrations below CMC decrease the solubility of Rho-123. For further studies, Rho-123 & SLS complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (DSC). Thermal analysis also proved the formation of complex between SLS & Rho-123. The P values were found to be significant (<0.05) except group comprising 0.0001% SLS, and that is because 0.0001% SLS is seems to be very low to affect the solubility or complexation of Rho-123.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / antagonists & inhibitors*
  • Animals
  • Biological Transport
  • Biomarkers
  • Male
  • Micelles
  • Rats
  • Rats, Sprague-Dawley
  • Rhodamine 123 / chemistry*
  • Rhodamine 123 / pharmacokinetics
  • Sodium Dodecyl Sulfate / chemistry*
  • Solubility

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Biomarkers
  • Micelles
  • Rhodamine 123
  • Sodium Dodecyl Sulfate