Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney

Biochem Biophys Res Commun. 1989 Oct 31;164(2):912-8. doi: 10.1016/0006-291x(89)91545-3.

Abstract

In human, the two subunits of gamma-glutamyl transpeptidase (GGT) arise from a common precursor encoded by a multigene family. Until now, a single specific coding sequence for this precursor (type I) has been identified in human placenta and liver. In the present study, we have isolated from a human kidney cDNA library, a GGT specific clone (0.8 Kb). The sequence of which (type II) i) covers the carboxy terminal part of the GGT precursor, ii) exhibits 22 point mutations and a 30 bp deletion as compared to the type I GGT sequence. The sequencing of a human genomic clone reveals that this type II GGT mRNA is encoded by a different gene than the type I GGT mRNA. Both type I and type II GGT mRNAs are expressed in human liver, while almost exclusively type II GGT mRNA is detected in human kidney.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression*
  • Genes*
  • Genomic Library
  • Humans
  • Isoenzymes / genetics*
  • Kidney / enzymology*
  • Liver / enzymology*
  • Molecular Sequence Data
  • RNA, Messenger / genetics*
  • Restriction Mapping
  • gamma-Glutamyltransferase / genetics*

Substances

  • Isoenzymes
  • RNA, Messenger
  • DNA
  • gamma-Glutamyltransferase

Associated data

  • GENBANK/M30474
  • GENBANK/M30475
  • GENBANK/M30476
  • GENBANK/M30477
  • GENBANK/M30478
  • GENBANK/M30479