Molecular cloning of murine intercellular adhesion molecule (ICAM-1)

EMBO J. 1989 Oct;8(10):2889-96.

Abstract

We have previously reported a murine lymphocyte surface antigen MALA-2 of approximately 95,000 Mr which is expressed mainly on activated lymphocytes. The rat monoclonal antibody YN1/1 that detects this antigen profoundly inhibits mixed lymphocyte response. We have now purified MALA-2 and determined its partial amino acid sequence. By using non-redundant synthetic oligonucleotides as probes, based on the amino acid sequence, we have isolated two full length cDNA clones encoding MALA-2. The two clones are identical except for the 5' end sequence. Expression of MALA-2 on transfected COS cells is only achieved with one of the two cDNA clones. The nucleotide sequence as well as the deduced amino acid sequence of MALA-2 display striking homology with those of the recently reported human intercellular adhesion molecule ICAM-1. All the unique features of the human ICAM-1, including its homology with the neural adhesion molecule NCAM, its internal repeat structure and the immunoglobulin-like structure, are found in MALA-2. Furthermore, purified MALA-2 crosslinked to a solid support binds Con A blasts that express LFA-1, the putative receptor for ICAM-1, and the binding can be blocked by YN1/1 antibody or antimurine LFA-1 antibody indicating a direct interaction of these molecules in cell adhesion. Therefore, we consider MALA-2 to be the murine homolog of human ICAM-1. Since ICAM-1 is known to be of primary importance in immune responses and inflammatory reactions, having a monoclonal antibody and a mouse model will provide the opportunity to study the functional role of ICAM-1 in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation / genetics*
  • Antigens, Differentiation / metabolism
  • Antigens, Surface / genetics*
  • Antigens, Surface / metabolism
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cell Adhesion Molecules / genetics*
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics
  • Humans
  • Intercellular Adhesion Molecule-1
  • Lymphocyte Function-Associated Antigen-1
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Receptors, Leukocyte-Adhesion / metabolism
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Tumor Necrosis Factor Receptor Superfamily, Member 7

Substances

  • Antigens, Differentiation
  • Antigens, Surface
  • Cell Adhesion Molecules
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Leukocyte-Adhesion
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Intercellular Adhesion Molecule-1
  • DNA