Chitin and chitosan preparation from marine sources. Structure, properties and applications

Mar Drugs. 2015 Mar 2;13(3):1133-74. doi: 10.3390/md13031133.


This review describes the most common methods for recovery of chitin from marine organisms. In depth, both enzymatic and chemical treatments for the step of deproteinization are compared, as well as different conditions for demineralization. The conditions of chitosan preparation are also discussed, since they significantly impact the synthesis of chitosan with varying degree of acetylation (DA) and molecular weight (MW). In addition, the main characterization techniques applied for chitin and chitosan are recalled, pointing out the role of their solubility in relation with the chemical structure (mainly the acetyl group distribution along the backbone). Biological activities are also presented, such as: antibacterial, antifungal, antitumor and antioxidant. Interestingly, the relationship between chemical structure and biological activity is demonstrated for chitosan molecules with different DA and MW and homogeneous distribution of acetyl groups for the first time. In the end, several selected pharmaceutical and biomedical applications are presented, in which chitin and chitosan are recognized as new biomaterials taking advantage of their biocompatibility and biodegradability.

Publication types

  • Review

MeSH terms

  • Acetylation
  • Animals
  • Aquatic Organisms / chemistry*
  • Chitin / chemistry*
  • Chitin / isolation & purification
  • Chitin / pharmacology
  • Chitosan / chemistry*
  • Chitosan / isolation & purification
  • Chitosan / pharmacology
  • Humans
  • Molecular Weight
  • Solubility


  • Chitin
  • Chitosan