Gene manipulation for regenerative studies using the Iberian ribbed newt, Pleurodeles waltl

Toshinori Hayashi; Takashi Takeuchi

doi:10.1007/978-1-4939-2495-0_23

Gene manipulation for regenerative studies using the Iberian ribbed newt, Pleurodeles waltl

Methods Mol Biol. 2015:1290:297-305. doi: 10.1007/978-1-4939-2495-0_23.

Authors

Toshinori Hayashi¹, Takashi Takeuchi

Affiliation

¹ Department of Biomedical Sciences, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, 683-8503, Tottori, Japan, toshi2@med.tottori-u.ac.jp.

PMID: 25740495
DOI: 10.1007/978-1-4939-2495-0_23

Abstract

Newts provide a unique model animal for regenerative studies. An experimental model system for molecular genetics in newts is needed in order to clarify the mechanisms of regeneration from the perspective of gene functions. We have identified that Iberian ribbed newt (Pleurodeles waltl) is a suitable model animal for such studies. Here we describe protocols for gene manipulation using Pleurodeles waltl.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Husbandry
Animals
Deoxyribonucleases, Type II Site-Specific / metabolism
Embryo, Nonmammalian / metabolism
Female
Gene Transfer Techniques
Genetic Engineering
Genetic Techniques*
Genetic Vectors / genetics
Insemination, Artificial
Male
Microinjections
Plasmids / genetics
Pleurodeles / genetics*
Pleurodeles / physiology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Regeneration / genetics*

Substances

RNA, Messenger
Deoxyribonucleases, Type II Site-Specific