Detection of enterovirus D68 in Canadian laboratories

Todd F Hatchette; Steven J Drews; Elsie Grudeski; Tim Booth; Christine Martineau; Kerry Dust; Richard Garceau; Jonathan Gubbay; Tim Karnauchow; Mel Krajden; Paul N Levett; Tony Mazzulli; Ryan R McDonald; Alan McNabb; Samira Mubareka; Robert Needle; Astrid Petrich; Susan Richardson; Candy Rutherford; Marek Smieja; Raymond Tellier; Graham Tipples; Jason J LeBlanc

doi:10.1128/JCM.03686-14

Detection of enterovirus D68 in Canadian laboratories

J Clin Microbiol. 2015 May;53(5):1748-51. doi: 10.1128/JCM.03686-14. Epub 2015 Mar 4.

Authors

Todd F Hatchette¹, Steven J Drews², Elsie Grudeski³, Tim Booth³, Christine Martineau⁴, Kerry Dust⁵, Richard Garceau⁶, Jonathan Gubbay⁷, Tim Karnauchow⁸, Mel Krajden⁹, Paul N Levett¹⁰, Tony Mazzulli¹¹, Ryan R McDonald¹⁰, Alan McNabb⁹, Samira Mubareka¹², Robert Needle¹³, Astrid Petrich¹⁴, Susan Richardson¹⁴, Candy Rutherford¹⁵, Marek Smieja¹⁵, Raymond Tellier¹⁶, Graham Tipples¹⁷, Jason J LeBlanc¹⁸

Affiliations

¹ Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Dalhousie University, Halifax, Nova Scotia, Canada Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada, University of Alberta, Edmonton, Alberta, Canada.
² Provincial Laboratory for Public Health (ProvLab), University of Alberta, Edmonton, Alberta, Canada Department Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
³ Enterovirus and Enteric Virus Laboratory, National Microbiology Laboratory (NML), Winnipeg, Manitoba, Canada.
⁴ Laboratoire de Santé Publique du Québec (LSPQ)/Institut National de Santé Publique du Québec (INSPQ), Québec, Canada.
⁵ Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
⁶ Centre Hospitalier Universitaire George L. Dumont, Moncton, New Brunswick, Canada.
⁷ Public Health Ontario and University of Toronto, Toronto, Ontario, Canada.
⁸ EORLA Regional Virology Laboratory, Children's Hospital of Eastern Ontario (CHEO), University of Ottawa, Ottawa, Ontario, Canada.
⁹ Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control (BCCDC), Vancouver, British Columbia, Canada.
¹⁰ Saskatchewan Disease Control Lab (SDCL), Ministry of Health, Regina, Saskatchewan, Canada.
¹¹ Department of Microbiology, Mount Sinai Hospital, University Health Network, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
¹² Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
¹³ Public Health Laboratory and Microbiology, St-John's, Newfoundland, Canada.
¹⁴ The Hospital for Sick Children, Toronto, Ontario, Canada.
¹⁵ St-Joseph's Healthcare, Hamilton, Ontario, Canada.
¹⁶ Provincial Laboratory for Public Health (ProvLab), University of Calgary, Calgary, Alberta, Canada.
¹⁷ Provincial Laboratory for Public Health (ProvLab), University of Alberta, Edmonton, Alberta, Canada Medical Microbiology and Immunology University of Alberta, Edmonton, Alberta, Canada.
¹⁸ Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Dalhousie University, Halifax, Nova Scotia, Canada Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada, University of Alberta, Edmonton, Alberta, Canada jason.leblanc@cdha.nshealth.ca.

Abstract

The recent emergence of a severe respiratory disease caused by enterovirus D68 prompted investigation into whether Canadian hospital and provincial laboratories can detect this virus using commercial and laboratory-developed assays. This study demonstrated analytical sensitivity differences between commercial and laboratory-developed assays for the detection of enterovirus D68.

MeSH terms

Canada
Clinical Laboratory Techniques / methods*
Diagnostic Tests, Routine / methods*
Enterovirus D, Human / isolation & purification*
Enterovirus Infections / diagnosis*
Enterovirus Infections / virology
Humans
Laboratory Proficiency Testing*
Respiratory Tract Infections / diagnosis*
Respiratory Tract Infections / virology
Sensitivity and Specificity