Characterization of eight VNTR loci by agarose gel electrophoresis

Genomics. 1989 Nov;5(4):915-24. doi: 10.1016/0888-7543(89)90134-1.

Abstract

Allelic frequencies and their confidence intervals were obtained for eight independent VNTR loci from a sample of more than 75 Utah Caucasians. Using high-resolution agarose gel electrophoresis, we were able to resolve alleles at the D17S5 locus that differed by only one repeating unit; it was therefore possible to name the alleles according to the number of repeating units each contained. Two a priori probabilities were calculated for each VNTR locus separately and for all eight loci jointly: (i) the "power of exclusion" for an alleged father/mother/child trio and for an alleged parent/child duo, and (ii) the "probability of matching" when two unrelated individuals or two siblings are genotyped.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Electrophoresis, Agar Gel
  • Female
  • Genotype
  • Humans
  • Male
  • Polymorphism, Restriction Fragment Length
  • Probability
  • Repetitive Sequences, Nucleic Acid*