Prenatal sex determination by DNA amplification from maternal peripheral blood

Lancet. 1989 Dec 9;2(8676):1363-5. doi: 10.1016/s0140-6736(89)91969-7.


The polymerase chain reaction was used to amplify a Y-specific repeat sequence in peripheral blood DNA samples from 19 pregnant women who had a gestational age of 9 to 41 weeks. Y-specific sequences were amplified from all 12 women who bore a male fetus but in none of 7 women who bore a female fetus. With stringent precautions against contamination, this technique may assist prenatal diagnosis of sex-linked genetic disorders.

MeSH terms

  • Blood*
  • Female
  • Fetus*
  • Gene Amplification*
  • Genetic Markers
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Pregnancy
  • Sex Determination Analysis*
  • Y Chromosome


  • Genetic Markers