Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix

J Nippon Med Sch. 2014;81(6):356-63. doi: 10.1272/jnms.81.356.


We developed a transplantable tissue-engineered skin equivalent composed of autologous cultured keratinocytes, fibroblasts, and a decellularized allogeneic dermis (acellular allogeneic dermal matrix; ADM) obtained from cadavers. In a process taking 3 weeks, cultured autologous keratinocytes from burn patients were expanded and then grown on ADMs. The tissue-engineered autologous skin equivalents (TESEs) were then transplanted in a one-stage procedure to the debrided third-degree burn wounds of 4 patients. The mean graft survival rate was 96%. Delayed graft loss and graft fragility were not observed. Histological and immunohistological findings indicated that the transplanted TESE had similar characteristics to normal human split-thickness skin grafts. These results suggest that the TESE using ADM can be used for permanent repair of full-thickness skin defects.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acellular Dermis*
  • Adult
  • Autografts / cytology*
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Skin, Artificial*
  • Tissue Engineering / methods*
  • Transplantation, Autologous