Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase

Curr Genet. 1989 Sep;16(3):153-8. doi: 10.1007/BF00391471.


The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to orotidine-5'-phosphate decarboxylases (ODCases) of other fungal species. The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida albicans / enzymology
  • Candida albicans / genetics*
  • Carboxy-Lyases / genetics*
  • Kluyveromyces / enzymology
  • Kluyveromyces / genetics
  • Molecular Sequence Data
  • Orotidine-5'-Phosphate Decarboxylase / genetics*
  • Poly A / genetics
  • RNA, Fungal / genetics
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Nucleic Acid


  • RNA, Fungal
  • RNA, Messenger
  • Poly A
  • Carboxy-Lyases
  • Orotidine-5'-Phosphate Decarboxylase

Associated data

  • GENBANK/X14198