Syneilesis palmata (Thunb.) Maxim. extract attenuates inflammatory responses via the regulation of TRIF-dependent signaling and inflammasome activation

J Ethnopharmacol. 2015 May 26:166:1-4. doi: 10.1016/j.jep.2015.02.056. Epub 2015 Mar 6.


Ethnopharmacological relevance: Syneilesis palmata (Thunb.) Maxim. (S. palmata, Asteraceae) is a traditional Korean therapeutic herb widely used to treat pain, arthritis, and other symptoms. This study provides the scientific basis for the anti-inflammatory effects of S. palmata extract (SP) in both in vitro and in vivo experimental models.

Materials and methods: Lipopolysaccharide (LPS)-stimulated murine macrophages were used to study the regulatory effect of SP on the inflammatory mediators in vitro. Bone marrow-derived macrophages were used to study the effects of SP on inflammasome activation. Escherichia coli-induced sepsis mouse model and LPS-induced endotoxin shock model were employed to study the effect of SP on in vivo efficacy.

Results: SP inhibited the LPS-stimulated release of proinflammatory mediators, such as nitric oxide and interleukin (IL)-6 in RAW 264.7 cells. SP treatment also attenuated IL-1β secretion via the inhibition of NLRP3 inflammasome activation induced by monosodium urate, ATP, and nigericin. Further, SP ameliorated the severity of NLRP3 inflammasome-mediated symptoms in LPS-induced endotoxin and E. coli-induced sepsis mouse models. Mechanistic studies revealed that inhibitory effects of SP were mediated through the regulation of TRIF-dependent signaling and inflammasome activation.

Conclusion: This study was the first to reveal mechanistic-based evidence substantiating the traditional claims of SP in the treatment of inflammation-related disorders, such as pain and arthritis.

Keywords: Asteraceae; Inflammasome; Inflammation; Sepsis; TRIF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology*
  • Asteraceae / chemistry*
  • Cell Line
  • Escherichia coli / pathogenicity
  • Female
  • Inflammasomes / metabolism*
  • Inflammation / drug therapy*
  • Inflammation / metabolism
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Nitric Oxide / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plants, Medicinal / chemistry
  • Sepsis / drug therapy
  • Sepsis / metabolism
  • Sepsis / microbiology
  • Shock, Septic / chemically induced
  • Shock, Septic / drug therapy
  • Shock, Septic / metabolism
  • Signal Transduction / drug effects*


  • Adaptor Proteins, Vesicular Transport
  • Anti-Inflammatory Agents
  • Inflammasomes
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • Plant Extracts
  • TICAM-1 protein, mouse
  • Nitric Oxide