10-oxo-12(Z)-octadecenoic acid, a linoleic acid metabolite produced by gut lactic acid bacteria, potently activates PPARγ and stimulates adipogenesis

Tsuyoshi Goto; Young-Il Kim; Tomoya Furuzono; Nobuyuki Takahashi; Kanae Yamakuni; Ha-Eun Yang; Yongjia Li; Ryuji Ohue; Wataru Nomura; Tatsuya Sugawara; Rina Yu; Nahoko Kitamura; Si-Bum Park; Shigenobu Kishino; Jun Ogawa; Teruo Kawada

doi:10.1016/j.bbrc.2015.02.154

10-oxo-12(Z)-octadecenoic acid, a linoleic acid metabolite produced by gut lactic acid bacteria, potently activates PPARγ and stimulates adipogenesis

Biochem Biophys Res Commun. 2015 Apr 17;459(4):597-603. doi: 10.1016/j.bbrc.2015.02.154. Epub 2015 Mar 5.

Authors

Affiliations

¹ Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji 611-0011, Japan; Research Unit for Physiological Chemistry, The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Japan. Electronic address: tgoto@kais.kyoto-u.ac.jp.
² Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji 611-0011, Japan.
³ Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji 611-0011, Japan; Research Unit for Physiological Chemistry, The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Japan.
⁴ Laboratory of Marine Bioproducts Technology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
⁵ Department of Food Science and Nutrition, University of Ulsan, Ulsan 680-749, South Korea.
⁶ Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
⁷ Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
⁸ Research Unit for Physiological Chemistry, The Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Japan; Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

PMID: 25749343
DOI: 10.1016/j.bbrc.2015.02.154

Abstract

Our previous study has shown that gut lactic acid bacteria generate various kinds of fatty acids from polyunsaturated fatty acids such as linoleic acid (LA). In this study, we investigated the effects of LA and LA-derived fatty acids on the activation of peroxisome proliferator-activated receptors (PPARs) which regulate whole-body energy metabolism. None of the fatty acids activated PPARδ, whereas almost all activated PPARα in luciferase assays. Two fatty acids potently activated PPARγ, a master regulator of adipocyte differentiation, with 10-oxo-12(Z)-octadecenoic acid (KetoA) having the most potency. In 3T3-L1 cells, KetoA induced adipocyte differentiation via the activation of PPARγ, and increased adiponectin production and insulin-stimulated glucose uptake. These findings suggest that fatty acids, including KetoA, generated in gut by lactic acid bacteria may be involved in the regulation of host energy metabolism.

Keywords: 10-oxo-12(Z)-octadecenoic acid; Adipocyte differentiation; Fatty acid; Gut lactic acid bacteria; Obesity; PPARγ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipogenesis / drug effects*
Animals
Energy Metabolism
Lactobacillus / metabolism*
Linoleic Acids / biosynthesis*
Linoleic Acids / pharmacology
Mice
NIH 3T3 Cells
PPAR gamma / metabolism*
Polymerase Chain Reaction

Substances

10-oxo-12-octadecenoic acid
Linoleic Acids
PPAR gamma