A novel chromatographic media: histidine-containing composite cryogels for HIgG separation from human serum

Methods Mol Biol. 2015:1286:221-31. doi: 10.1007/978-1-4939-2447-9_18.

Abstract

Histidine-containing microspheres (HCM) with 2 μm in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.

MeSH terms

  • Acrylamides / chemistry
  • Adsorption
  • Buffers
  • Chromatography, Affinity / methods*
  • Cryogels / chemistry*
  • Histidine / chemistry*
  • Humans
  • Hydrogen-Ion Concentration
  • Immunoglobulin G / blood
  • Immunoglobulin G / chemistry*
  • Immunoglobulin G / isolation & purification*
  • Methacrylates / chemistry
  • Microspheres
  • Polyhydroxyethyl Methacrylate / chemistry
  • Water / chemistry

Substances

  • Acrylamides
  • Buffers
  • Cryogels
  • Immunoglobulin G
  • Methacrylates
  • Water
  • Polyhydroxyethyl Methacrylate
  • Histidine
  • hydroxyethyl methacrylate
  • N,N'-methylenebisacrylamide