Lysobacter lycopersici sp. nov., isolated from tomato plant Solanum lycopersicum

Antonie Van Leeuwenhoek. 2015 May;107(5):1261-70. doi: 10.1007/s10482-015-0419-1. Epub 2015 Mar 7.

Abstract

A Gram-stain negative, aerobic, rod-shaped and non-spore-forming bacterium, CC-Bw-6(T) was isolated from chopped tomato stems. Analysis of the 16S rRNA gene sequence of the strain CC-Bw-6(T) showed its affiliation with the genus Lysobacter within the class Gammaproteobacteria. Strain CC-Bw-6(T) was found to be most closely related to Lysobacter panaciterrae KCTC 12601(T) (97.0%) and Lysobacter daecheongensis KCTC 12600(T) (96.8%), and showed lower similarity (<96.5%) to other Lysobacter species. DNA-DNA relatedness between strain CC-Bw-6(T) and L. panaciterrae KCTC 12601(T) was 10.8%, the G+C content of the genomic DNA is 69.9 mol%. Strain CC-Bw-6(T) was determined to possess C(11:0) iso, C(11:0) iso 3OH, C(14:0) iso, C(15:0) anteiso, C(15:1) ω5c, C(16:1) ω5c, C(16:0), C(15:0) iso, C(16:0) iso, C(17:0) iso and C(16:0) 10-methyl/C(17:1) iso ω9c as predominant fatty acids. The major polar lipid profile consists of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipids. The predominant polyamine is spermidine, and ubiquinone-8 is the predominant respiratory quinone. Analysis of phenotypic, geno- and phylogenetic characteristics revealed a distinct taxonomic position of strain CC-Bw-6(T) with respect to other Lysobacter species. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, we propose a novel species with the name Lysobacter lycopersici sp. nov. The type strain is CC-Bw-6(T) (=BCRC 80612(T) = JCM 19164(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Lysobacter / classification*
  • Lysobacter / genetics
  • Lysobacter / isolation & purification*
  • Lysobacter / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Solanum lycopersicum / microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KC820654