Distinct human stem cell populations in small and large intestine

PLoS One. 2015 Mar 9;10(3):e0118792. doi: 10.1371/journal.pone.0118792. eCollection 2015.

Abstract

The intestine is composed of an epithelial layer containing rapidly proliferating cells that mature into two regions, the small and the large intestine. Although previous studies have identified stem cells as the cell-of-origin for intestinal epithelial cells, no studies have directly compared stem cells derived from these anatomically distinct regions. Here, we examine intrinsic differences between primary epithelial cells isolated from human fetal small and large intestine, after in vitro expansion, using the Wnt agonist R-spondin 2. We utilized flow cytometry, fluorescence-activated cell sorting, gene expression analysis and a three-dimensional in vitro differentiation assay to characterize their stem cell properties. We identified stem cell markers that separate subpopulations of colony-forming cells in the small and large intestine and revealed important differences in differentiation, proliferation and disease pathways using gene expression analysis. Single cells from small and large intestine cultures formed organoids that reflect the distinct cellular hierarchy found in vivo and respond differently to identical exogenous cues. Our characterization identified numerous differences between small and large intestine epithelial stem cells suggesting possible connections to intestinal disease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Biomarkers / metabolism*
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Proliferation
  • Cell Separation
  • Cells, Cultured
  • Flow Cytometry
  • Gene Expression Profiling
  • Humans
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Intestine, Large / cytology
  • Intestine, Large / embryology*
  • Intestine, Large / metabolism
  • Intestine, Small / cytology
  • Intestine, Small / embryology*
  • Intestine, Small / metabolism
  • Stem Cells / cytology*
  • Stem Cells / physiology

Substances

  • Biomarkers
  • Intercellular Signaling Peptides and Proteins