Broad-range inhibition of enterovirus replication by OSW-1, a natural compound targeting OSBP

Antiviral Res. 2015 May;117:110-4. doi: 10.1016/j.antiviral.2015.02.013. Epub 2015 Mar 6.

Abstract

Enteroviruses, e.g., polio-, coxsackie- and rhinoviruses, constitute a large genus within the Picornaviridae family of positive-strand RNA viruses and include many important pathogens linked to a variety of acute and chronic diseases. Despite their huge medical and economic impact, no approved antiviral therapy is yet available. Recently, the oxysterol-binding protein (OSBP) was implicated as a host factor for enterovirus replication. Here, we investigated the antiviral activity of the natural compound OSW-1, a ligand of OSBP that is under investigation as an anti-cancer drug. OSW-1 potently inhibited the replication of all enteroviruses tested, with IC50 values in the low nanomolar range, acted at the genome replication stage and was effective in all tested cell types of three different species. Importantly, OSBP overexpression rescued viral replication, demonstrating that the antiviral effect of OSW-1 is due to targeting OSBP. Together, we here report the anti-enterovirus activity of the natural anti-cancer compound OSW-1.

Keywords: Antiviral; Coxsackievirus; Enterovirus; Oxysterol-binding protein; Replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Cell Line
  • Chlorocebus aethiops
  • Cholestenones / pharmacology*
  • Enterovirus / drug effects*
  • Enterovirus / growth & development
  • Genome, Viral / drug effects
  • HeLa Cells
  • Humans
  • Ligands
  • Receptors, Steroid / metabolism*
  • Saponins / pharmacology*
  • Vero Cells
  • Viral Load / drug effects
  • Virus Replication / drug effects*

Substances

  • Antiviral Agents
  • Cholestenones
  • Ligands
  • Receptors, Steroid
  • Saponins
  • oxysterol binding protein
  • OSW 1