Complete sequence of a bla(KPC)-harboring cointegrate plasmid isolated from Escherichia coli

Antimicrob Agents Chemother. 2015 May;59(5):2956-9. doi: 10.1128/AAC.00041-15. Epub 2015 Mar 9.

Abstract

Horizontal transfer of bla(KPC)-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla(KPC)-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how bla(KPC) can be spread from a nonconjugative plasmid through cointegration.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Escherichia coli / genetics*
  • Klebsiella pneumoniae / genetics*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics
  • beta-Lactamases / genetics

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase

Associated data

  • GENBANK/KP345882