MicroRNA-130a inhibits cell proliferation, invasion and migration in human breast cancer by targeting the RAB5A

Int J Clin Exp Pathol. 2015 Jan 1;8(1):384-93. eCollection 2015.

Abstract

MiR-130a has been demonstrated to play important roles in many types of cancers. Nevertheless, its biological function in breast cancer remains largely unknown. In this study, we found that the expression level of miR-130a was down-regulated in breast cancer tissues and cells. Overexpression of miR-130a was able to inhibit cell proliferation, invasion and migration in MCF7 and MDA-MB-435 cells. With the bioinformatics analysis, we further identified that RAB5A was a directly target of miR-130a, and its mRNA and protein level was negatively regulated by miR-130a. Immunohistochemistry verified RAB5A was upregulated in breast cancer tissues. Therefore, the data reported here demonstrate that miR-130a is an important tumor suppressor in breast cancer, and imply that miR-130a/RAB5A axis have potential as therapeutic targets for breast cancer.

Keywords: RAB5A; breast cancer; cell invasion; cell migration; cell proliferation; miR-130a.

MeSH terms

  • Blotting, Western
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Movement* / genetics
  • Cell Proliferation* / genetics
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Tumor Suppressor
  • Humans
  • Immunohistochemistry
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • Real-Time Polymerase Chain Reaction
  • Transfection
  • rab5 GTP-Binding Proteins / biosynthesis*
  • rab5 GTP-Binding Proteins / genetics

Substances

  • MIRN130 microRNA, human
  • MicroRNAs
  • RAB5C protein, human
  • rab5 GTP-Binding Proteins