Identification and characterization of a potent anticancer fraction from the leaf extracts of Moringa oleifera L

Indian J Exp Biol. 2015 Feb;53(2):98-103.

Abstract

Anticancer potential of Moringa oleifera L. extracts have been well established. However, there are no reports on the isolated molecules/fractions from these extracts which are responsible for the anticancer/cytotoxic activity. Thus, in the present study, we explored the same. The n-hexane, chloroform, ethyl acetate, methanol extracts of the M. oleifera leaves and 15 fractions (F1 to F15) of ethyl acetate extract were evaluated for their in vitro and in vivo anticancer activity using Hep-2 cell lines and Dalton's lymphoma ascites model in mice, respectively. Among the tested samples, the F1 fraction showed potential cytotoxic effect in Hep-2 cell lines with a CTC50 value of 12.5 ± 0.5 μg/ml. In vivo studies with the doses 5 and 10 mg/kg, p.o. demonstrated significant reduction in body weight and increased the mean survival time compared to the control group. These results were also comparable to the standard, 5-Fluorouracil, treated animals. We have also successfully isolated and characterized the anticancer fraction, F1 from the leaves of M. oleifera L.

MeSH terms

  • Acetates / chemistry
  • Animals
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Chemical Fractionation / methods
  • Chlorocebus aethiops
  • Chloroform / chemistry
  • Female
  • Hep G2 Cells
  • Hexanes / chemistry
  • Humans
  • Inhibitory Concentration 50
  • Male
  • Methanol / chemistry
  • Moringa oleifera / chemistry*
  • Neoplasms, Experimental / drug therapy
  • Neoplasms, Experimental / pathology
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Survival Analysis
  • Time Factors
  • Vero Cells

Substances

  • Acetates
  • Antineoplastic Agents
  • Hexanes
  • Plant Extracts
  • n-hexane
  • ethyl acetate
  • Chloroform
  • Methanol