Organelles maintain spindle position in plant meiosis

Nat Commun. 2015 Mar 11;6:6492. doi: 10.1038/ncomms7492.

Abstract

Accurate positioning of spindles is a critical aspect of cell division as it ensures that each daughter cell contains a single nucleus. In many flowering plants, two meiotic chromosome separations occur without intervening cytokinesis, resulting in two spindles in one cell during the second division. Here we report a detailed examination of two mutants, jason (jas) and parallel spindle1 (ps1), in which disturbed spindle position during male meiosis II results in the incorporation of previously separated chromosome groups into a single cell. Our study reveals that an organelle band provides a physical barrier between the two spindles. The loss of a single protein, JAS, from this organelle band leads to its disruption and a random movement of the spindles. JAS is largely associated with vesicles in the organelle band, revealing a role for vesicles in plant meiosis and that cytoplasmic events maintain spindle position during the chromosome division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis / ultrastructure
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Chromosome Segregation
  • Cytokinesis
  • Gene Expression
  • Golgi Apparatus / metabolism
  • Golgi Apparatus / ultrastructure
  • Meiosis
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Mitochondria / metabolism
  • Mitochondria / ultrastructure
  • Mutation
  • Peroxisomes / metabolism
  • Peroxisomes / ultrastructure
  • Plant Cells / metabolism
  • Plant Cells / ultrastructure
  • Plant Roots / metabolism
  • Plant Roots / ultrastructure
  • Plants, Genetically Modified
  • Sex Factors
  • Spindle Apparatus / metabolism*
  • Spindle Apparatus / ultrastructure
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism

Substances

  • Arabidopsis Proteins
  • Jason protein, Arabidopsis
  • PS1 protein, Arabidopsis
  • Trans-Activators