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. 2015 Mar 11;10(3):e0120972.
doi: 10.1371/journal.pone.0120972. eCollection 2015.

Vitamin B6 in Plasma and Cerebrospinal Fluid of Children

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Free PMC article

Vitamin B6 in Plasma and Cerebrospinal Fluid of Children

Monique Albersen et al. PLoS One. .
Free PMC article

Abstract

Background: Over the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce.

Materials and methods: B6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF) of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem mass spectrometry. For ethical reasons, CSF samples could not be obtained from healthy children. The influence of sex, age, epilepsy and treatment with anti-epileptic drugs, were investigated.

Results: The B6 vitamer composition of plasma (pyridoxal phosphate (PLP) > pyridoxic acid > pyridoxal (PL)) differed from that of CSF (PL > PLP > pyridoxic acid > pyridoxamine). Strong correlations were found for B6 vitamers in and between plasma and CSF. Treatment with anti-epileptic drugs resulted in decreased concentrations of PL and PLP in CSF.

Conclusion: We provide concentrations of all B6 vitamers in plasma and CSF of children with intellectual disability (±epilepsy), which can be used in the investigation of known and novel disorders associated with vitamin B6 metabolism as well as in monitoring of the biochemical effects of treatment with vitamin B6.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Human vitamin B6 metabolism.
PDXK = pyridoxal kinase. PDXP = vitamin B6-specific phosphatase. PNPO = pyridox(am)ine phosphate oxidase.
Fig 2
Fig 2. A. Correlation (Spearman’s rho, p-value) between PLP and PL in plasma (ρ = 0.622, p<0.001) of children (n = 42).
B. Correlation (Spearman’s rho, p-value) between PL in CSF and PL in plasma (ρ = 0.806, p<0.001) of children (n = 35).
Fig 3
Fig 3. Concentrations of PL, PLP and PM in CSF of children using one or more AEDs (n = 51; from both the original and additional sets) compared to children not using any AEDs (n = 41).
Median concentrations of PL, PLP and PM were lower in CSF of children with AED-treated epilepsy than in CSF of children not using any AEDs.

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