Novel biological activity of ameloblastin in enamel matrix derivative

J Appl Oral Sci. Jan-Feb 2015;23(1):49-55. doi: 10.1590/1678-775720140291.

Abstract

Objective: Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration. However, the effects of EMD on gingival epithelial cells during regeneration of periodontal tissues are unclear. In this in vitro study, we purified ameloblastin from EMD and investigated its biological effects on epithelial cells.

Material and methods: Bioactive fractions were purified from EMD by reversed-phase high-performance liquid chromatography using hydrophobic support with a C18 column. The mouse gingival epithelial cell line GE-1 and human oral squamous cell carcinoma line SCC-25 were treated with purified EMD fraction, and cell survival was assessed with a WST-1 assay. To identify the proteins in bioactive fractions of EMD, we used proteome analysis with two-dimensional gel electrophoresis followed by identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

Results: Purified fractions from EMD suppressed proliferation of GE-1 and SCC-25. LC-MS/MS revealed that ameloblastin in EMD is the component responsible for inhibiting epithelial cell proliferation. The inhibitory effect of ameloblastin on the proliferation of GE-1 and SCC-25 was confirmed using recombinant protein.

Conclusion: The inhibitory effects of EMD on epithelial cell proliferation are caused by the biological activities of ameloblastin, which suggests that ameloblastin is involved in regulating epithelial downgrowth in periodontal tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Dental Enamel Proteins / pharmacology*
  • Electrophoresis, Gel, Two-Dimensional
  • Epithelial Cells / cytology
  • Epithelial Cells / drug effects*
  • Gingiva / cytology
  • Gingiva / drug effects
  • Guided Tissue Regeneration, Periodontal / methods
  • Humans
  • Mice
  • Periodontitis / drug therapy
  • Periodontium / drug effects*
  • Reference Values
  • Reproducibility of Results
  • Silver Staining
  • Time Factors

Substances

  • AMBN protein, human
  • Dental Enamel Proteins
  • enamel matrix proteins