The Ret receptor regulates sensory neuron dendrite growth and integrin mediated adhesion

Elife. 2015 Mar 12:4:e05491. doi: 10.7554/eLife.05491.


Neurons develop highly stereotyped receptive fields by coordinated growth of their dendrites. Although cell surface cues play a major role in this process, few dendrite specific signals have been identified to date. We conducted an in vivo RNAi screen in Drosophila class IV dendritic arborization (C4da) neurons and identified the conserved Ret receptor, known to play a role in axon guidance, as an important regulator of dendrite development. The loss of Ret results in severe dendrite defects due to loss of extracellular matrix adhesion, thus impairing growth within a 2D plane. We provide evidence that Ret interacts with integrins to regulate dendrite adhesion via rac1. In addition, Ret is required for dendrite stability and normal F-actin distribution suggesting it has an essential role in dendrite maintenance. We propose novel functions for Ret as a regulator in dendrite patterning and adhesion distinct from its role in axon guidance.

Keywords: D. melanogaster; cell adhesion; cell-matrix interactions; dendrite development; extracellular matrix; neuroscience; patterning; receptors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Cell Adhesion
  • Cell Membrane / metabolism
  • Dendrites / metabolism*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / metabolism*
  • Epithelial Cells / metabolism
  • Extracellular Matrix / metabolism
  • Gene Knockdown Techniques
  • Integrins / metabolism*
  • Mutation / genetics
  • Protein Binding
  • Proto-Oncogene Proteins c-ret / metabolism*
  • RNA Interference
  • Sensory Receptor Cells / cytology*
  • Sensory Receptor Cells / metabolism*
  • rac1 GTP-Binding Protein / metabolism


  • Actins
  • Drosophila Proteins
  • Integrins
  • Proto-Oncogene Proteins c-ret
  • Ret protein, Drosophila
  • rac1 GTP-Binding Protein