Biocompatibility of calcium phosphate bone cement with optimized mechanical properties

J Biomed Mater Res B Appl Biomater. 2016 Feb;104(2):308-15. doi: 10.1002/jbm.b.33370. Epub 2015 Mar 12.


The broad aim of this work was to investigate and optimize the properties of calcium phosphate bone cements (CPCs) for use in vertebroplasty to achieve effective primary fixation of spinal fractures. The incorporation of collagen, both bovine and from a marine sponge (Chondrosia reniformis), into a CPC was investigated. The biological properties of the CPC and collagen-CPC composites were assessed in vitro through the use of human bone marrow stromal cells. Cytotoxicity, proliferation, and osteoblastic differentiation were evaluated using lactate dehydrogenase, PicoGreen, and alkaline phosphatase activity assays, respectively. The addition of both types of collagen resulted in an increase in cytotoxicity, albeit not to a clinically relevant level. Cellular proliferation after 1, 7, and 14 days was unchanged. The osteogenic potential of the CPC was reduced through the addition of bovine collagen but remained unchanged in the case of the marine collagen. These findings, coupled with previous work showing that incorporation of marine collagen in this way can improve the physical properties of CPCs, suggest that such a composite may offer an alternative to CPCs in applications where low setting times and higher mechanical stability are important.

Keywords: bioactivity; biocompatibility; calcium phosphate bone cement; collagen; optimized mechanical properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Bone Cements* / chemistry
  • Bone Cements* / pharmacology
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Calcium Phosphates* / chemistry
  • Calcium Phosphates* / pharmacology
  • Cattle
  • Cells, Cultured
  • Collagen* / chemistry
  • Collagen* / pharmacology
  • Humans
  • Male
  • Porifera / chemistry*
  • Stromal Cells / cytology
  • Stromal Cells / metabolism


  • Bone Cements
  • Calcium Phosphates
  • Collagen
  • calcium phosphate