Analysis of volatile phenols in alcoholic beverage by ethylene glycol-polydimethylsiloxane based stir bar sorptive extraction and gas chromatography-mass spectrometry

J Chromatogr A. 2015 Apr 17;1390:22-7. doi: 10.1016/j.chroma.2015.02.064. Epub 2015 Feb 26.

Abstract

An ethylene glycol (EG)/polydimethylsiloxane (PDMS) copolymer based stir bar sorptive extraction (SBSE)-GC-MS method was developed for the analysis of volatile phenols (4-ethylphenol, 4-vinylphenol, 4-ethylguaiacol, and 4-vinylguaiacol) in alcoholic beverages. The beverage samples were diluted with phosphate buffer (1M, pH 7) and extracted with an EG/PDMS stir bar. Volatile phenols were thermally desorbed and analyzed by GC-MS. Parameters affecting extraction efficiency were studied including ionic strength, pH, extraction time, ethanol content and nonvolatile matrix. Good correlation coefficients with R(2) in the range of 0.994-0.999 were obtained for volatile phenol concentration of 5-500μg/L. Recovery for all phenols were from 95.7% to 104.4% in a beer matrix and 81.4% to 97.6% in a wine matrix. The method had a standard deviation less than 5.8% for all volatile phenols. The limit of quantification (LOQs) in beer samples was lower than 3μg/L. The method was further applied to analyze the concentrations of volatile phenols in beer, wine and other alcoholic beverage samples.

Keywords: Beer; EG/PDMS phase; SBSE-GC–MS; Volatile phenols; Wine.

MeSH terms

  • Alcoholic Beverages / analysis*
  • Beer / analysis
  • Dimethylpolysiloxanes / chemistry*
  • Ethanol / analysis
  • Ethylene Glycol / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Guaiacol / analogs & derivatives
  • Guaiacol / analysis
  • Hydrogen-Ion Concentration
  • Osmolar Concentration
  • Phenols / analysis*
  • Wine / analysis

Substances

  • Dimethylpolysiloxanes
  • Phenols
  • Ethanol
  • Guaiacol
  • 4-vinylguaiacol
  • 4-ethylphenol
  • 4-ethylguaiacol
  • Ethylene Glycol
  • 4-vinylphenol