Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium

Exp Biol Med (Maywood). 2015 May;240(5):585-92. doi: 10.1177/1535370214556946. Epub 2015 Mar 11.

Abstract

Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothelial cells and cardiomyocyte-like differentiation of BMSCs in vitro. Four weeks after transplantation of fibrin patch loaded with gene-modified BMSCs, IGF-1 overexpression could successfully promote angiogenesis, inhibit remodeling, increase grafted cell survival and reduce apoptosis. In conclusion, the integrated strategy, which combined fibrin patch with IGF-1 gene modified BMSCs, could promote the histological cardiac repair for a clinically relevant porcine model of I/R myocardium injury.

Keywords: Insulin-like growth factor-1; cardiac repair; fibrin patch; mesenchymal stem cells; myocardial infarction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA Primers
  • Disease Models, Animal
  • Fibrin / administration & dosage*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Insulin-Like Growth Factor I / genetics*
  • Myocardial Ischemia / therapy*
  • Stem Cell Transplantation*
  • Swine

Substances

  • DNA Primers
  • Insulin-Like Growth Factor I
  • Fibrin