Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse

Lili Jia; Geisa A L Gonçalves; Yusaku Takasugi; Yutaro Mori; Shuhei Noda; Tsutomu Tanaka; Hirofumi Ichinose; Noriho Kamiya

doi:10.1016/j.biortech.2015.02.041

Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse

Bioresour Technol. 2015 Jun:185:158-64. doi: 10.1016/j.biortech.2015.02.041. Epub 2015 Feb 17.

Authors

Lili Jia¹, Geisa A L Gonçalves², Yusaku Takasugi³, Yutaro Mori⁴, Shuhei Noda⁵, Tsutomu Tanaka⁶, Hirofumi Ichinose⁷, Noriho Kamiya⁸

Affiliations

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: lilijia@mail.cstm.kyushu-u.ac.jp.
² Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: geisa@mail.cstm.kyushu-u.ac.jp.
³ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: y_takasugi@mail.cstm.kyushu-u.ac.jp.
⁴ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address: forest@mail.cstm.kyushu-u.ac.jp.
⁵ Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address: shuhei.noda@riken.jp.
⁶ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan. Electronic address: tanaka@kitty.kobe-u.ac.jp.
⁷ Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: ichinose@agr.kyushu-u.ac.jp.
⁸ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, Fukuoka 819-0388, Japan. Electronic address: nori_kamiya@mail.cstm.kyushu-u.ac.jp.

PMID: 25768418
DOI: 10.1016/j.biortech.2015.02.041

Abstract

The effect of pretreatment with peracetic acid (PAA) or an ionic liquid (1-ethyl-3-methylimidazolium acetate, [Emim][OAc]) on the synergism between endoglucanase and endoxylanase in the hydrolysis of bagasse was investigated. An endoglucanase, Cel6A, with a carbohydrate-binding module (CBM) and two endoxylanases, XynZ-C without a CBM and Xyn11A with an intrinsic xylan/cellulose binding module (XBM), were selected. The hemicellulose content, especially arabinan, and the cellulose crystallinity of bagasse were found to affect the cellulase-xylanase synergism. More specifically, higher synergism (above 3.4) was observed for glucan conversion, at low levels of arabinan (0.9%), during the hydrolysis of PAA pretreated bagasse. In contrast, [Emim][OAc] pretreated bagasse, showed lower cellulose crystallinity and achieved higher synergism (over 1.9) for xylan conversion. Ultimately, the combination of Cel6A and Xyn11A resulted in higher synergism for glucan conversion than the combination of Cel6A with XynZ-C, indicating the importance of the molecular architecture of enzymes for metabolic synergism.

Keywords: Endoglucanase; Endoxylanase; Hemicellulose; Pretreatment; Synergism.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Cellulase / chemistry*
Cellulose / chemistry*
Endo-1,4-beta Xylanases / chemistry*
Enzyme Activation
Glucans / chemical synthesis
Hydrolysis
Ionic Liquids / chemistry*
Multienzyme Complexes / chemistry
Peracetic Acid / chemistry*
Substrate Specificity
Xylans / chemical synthesis

Substances

Glucans
Ionic Liquids
Multienzyme Complexes
Xylans
Cellulose
bagasse
Cellulase
Endo-1,4-beta Xylanases
Peracetic Acid